Vasco and Cruzeiro will go head to head at São Januário on 29 August, with both clubs announcing their first teams ahead of the match.
The two sides are keeping close tabs on the Copa do Brasil, giving added importance to the game.
Preparations were visible to supporters at the stadium before kickoff, as both squads completed their routines.
Managers Pedro Emanuel and Artur Jorge renew a rivalry that stretches back to Portugal, with this fixture being the first time they have clashed on Brazilian soil.
Broadcast details and match odds for the game are listed on the usual channels.
The fixture writes a new page in the long history between these two clubs, and both sets of supporters are expected to turn out in numbers as the managers look to gain the upper hand.
Both sides are in contention for a place in the next round, ensuring the final score all the more significant.